ABSTRACT
Objective To explore the protective effects and mechanism of quercetin against the oxidative damage to renal cell.Methods Using the model of renal cell lines HK-2,hydrogen peroxide (H2 O2 )induced oxidative damage on renal cells.On the basis of the effects of quercetin on HK-2 cells viability observed by CCK8,the alteration of cell signaling pathway after treating with quercetin was detected by immunoblotting.The glutathione (GSH)in the cell was also detected. Results It was found that H2 O2 can induce the oxidative damage in HK2 cells.Compared to control group,the cell viability decreased to 34. 9%.While pretreated with quercetin (10 μM~40 μM),the viability was between 40% and 70%.Quercetin decreased reactive oxygen species (ROS)to 1 10%-130% in HK2 cells which were induced by H2 O2 to 153%.Additionally,quercetin increased the content of anti-oxidant enzyme such as GPX1,SOD2 and GSH.Moreover, quercetin can promote the degradation of p-AMPK and LC3 and decrease p-mTOR,as well as inhibit the activation of BAX and caspase 3.Conclusion Quercetin has a protective effect on renal cell induced by oxidative damage,and the mechanism may be related to antioxidant effects and promoting autophagy.